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FREEZE ETCHING
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DeCS
Descriptor
English
:
Freeze Etching
Descriptor
Spanish
:
Grabado por Congelación
Descriptor
Portuguese
:
Técnica de Congelamento e Réplica
Tree Number:
E01.370.225.500.620.620.260.400
E01.370.225.750.600.620.260.400
E05.200.500.620.620.260.400
E05.200.750.600.620.260.400
Definition
English
:
A replica technique in which
cells
are frozen to a very low
temperature
and cracked with a knife blade to expose the interior surfaces of the
cells
or cell
membranes
. The cracked cell surfaces are then freeze-dried to expose their constituents. The surfaces are now ready for shadowing to be viewed using an electron microscope. This method differs from freeze-fracturing in that no cryoprotectant is used and, thus, allows for the
sublimation
of
water
during the freeze-drying process to etch the surfaces.
Indexing Annotation
English
:
do not confuse with
FREEZE FRACTURING
(wherein a cryoprotectant is used)
History Note
English
:
73(69)
Allowable Qualifiers
English
:
CL
classification
EC
economics
ES
ethics
HI
history
IS
instrumentation
MT
methods
ST
standards
SN
statistics & numerical data
TD
trends
UT
utilization
VE
veterinary
Record Number:
5749
Unique Identifier:
D005613
Occurrence in VHL
:
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DeCS
CID-10
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